Equipment

Cytek Aurora

Description: Cell Analyzer

67 Channel System + Plate Loader
5 Laser UV/V/B/YG/R
64 Fluorescence Channels, 3 Scattering Channels (FSC, blue laser SSC, violet laser SSC)
Spectraflo Software

Optics
Lasers: Base model three-laser configuration: 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW
Beam Geometry: Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.
Emission Optics
Emission Collection: Fused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.
Forward and Side Scatter Detection:  
FSC: high-performance semiconductor detector with 488nm bandpass filter 
SSC: two high-performance semiconductor detectors with 405nm and 488nm bandpass filters

Fluorescence Detectors: Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-829 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers

Standard Optical Configuration:
Violet detector module: 16 channels unevenly spaced bandwidth from 420-829 nm. 
Blue detector module: 14 channels unevenly spaced bandwidth from 498-829 nm. 
Red detector module: 8 channels unevenly spaced bandwidth from 652-829 nm.
4 and 5 Laser Options: Yellow-Green detector module: 10 channels unevenly spaced bandwidth from 567-829 nm.  
Ultraviolet detector module: 16 channels unevenly spaced bandwidth from 365-829 nm.
Fluidics

Sample Flow Rates: 
Low: 15 µL/min
Medium: 30 µL/min
High: 60 µL/min, Plate high-throughput mode: 100 µL/min
Fluidic Modes: Long clean, SIT flush, Purge filter, Clean flow cell
Manual Sample Input Formats: 12×75 mm polystyrene and polypropylene tubes
Standard Fluidic Reservoirs: 4L fluid container set with level-sensing provided. Compatible with 20 L sheath and waste cubitainers.
Volumetric Sensor: Volumetric measurement during sample recording enables calculation of counts per µL for any gated population.  
Plate Loader Options
Plate stage temperature: 4-30°C (AMS only)
High Throughput Speed: ASL Loader: 27 min for 96-well plate AMS Loader: 35 min for 96-well plate
Input Compatibility: 
ASL Loader: 27 min for 96-well plate
AMS Loader: 35 min for 96-well plate
Plate Loader Carryover
Default mode: ≤0.3%, Low Carryover mode: ≤0.1%, High Throughput mode: ≤1%
Performance
Fluorescence Linearity: FITC R2 ≥0.995 / PE R2 ≥0.995
Forward and Side Scatter Resolution: Performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Carryover: ≤0.1%
Data Acquisition Rate: 35,000 events/s (Three laser system)
Software
Live unmixing during acquisition
Developed specifically to streamline assay setup, data acquisition, and file export 
Automated QC module 
Autofluorescence extraction 
Raw and Unmixed FCS 3.1 files 

Labconco Freezone Triad

Description: Benchtop Freeze Dryer (Lyophilizer)

Stainless steel collector coil capable of removing 1.84 L of water in 24 hours and holding 2.5 L of ice before defrosting* 

Two 1/3 hp HCFC/CFC-free refrigeration compressors cool the collector to -85° C (-121° F) and work in concert with a 1000-watt heater to cool and heat fluid medium circulating through channels in the shelf. 

Not for use with samples containing methanol or ethanol 

Processing shelf, 12.4″ w x 14.5″ d (31.5 x 36.8 cm). Spacing between the shelf and top of the chamber accommodates containers with stoppers up to 148 mm maximum height 

One probe for monitoring the temperature of the sample in the chamber 

Pirani vacuum sensor 

Temperature ramp and hold programming from -55° C to +50° C (-67° F to +122° F) and -75° C (-103° F) during pre-freezing. Controls temperature to ±1° C of set point. Memory to store 30 programs with 16 segments each including a number of steps, ramp rate, shelf temperature, hold time, and vacuum level 

Full color, 5″ capacitive touch screen with Lyo-Works OS: – Real time display of collector temperature and vacuum level – Creation and storage for up to 30 programs with up to 16 steps each. Ability to set ramp rate, shelf temperature, shelf temperature time, and vacuum set point per step – Manual start-up buttons for collector and shelf temperature control and vacuum pull down – Alerts, diagnostic tests, user manual and defrost control – Data logging stores and displays data in table or graph format, and may be exported via USB or Ethernet – Settings include language (English, Spanish, French, German, or Italian), time, temperature (° C or ° F) and vacuum (mBar, Torr or Pa) – Alerts can be emailed to user-entered addresses via Ethernet connection – Vacuum pump maintenance alert – Security lock-out 

Pneumatic stoppering mechanism with diaphragm and plate. No compressed gas is required 

Stoppering control knob, vacuum release control knob, and 1/8″ OD user-supplied sterile/inert gas backfill port 

Four left side-mounted neoprene valves with molded plastic knobs and fittings that accommodate 1/2″ and 3/4″ flask adapters

Electrical receptacle for vacuum pump 

Hot gas defrost with the stainless steel drain pan, silicone drain hose and auto shut off at +60° C (+140° F) 

Vacuum control valve 

Vacuum break valve 

Stainless steel and powder-coated steel exterior 

Clear acrylic door, 1″ thick, with silicon gasket and latch 

3/4″ OD vacuum connection. 36″ long, 3/4″ ID vacuum hose with two clamps

Serum Bottle Capacity 

FreeZone® Triad® Benchtop Freeze Dryers Specifications *Freeze drying rate will be lower for samples other than plain water. For optimum performance, room temperature should be 21° C (70° F) or colder.

Heidolph HEI-VAP Expert Control ML/G3B

Description: Rotary Evaporator
Rotation Speed Indicator: 5″ LCD color graphic display 
Permissible Ambient Conditions: 5 – 31 °C at 80 % rel. humidity 32 – 40 °C decreasing lineary up to max 50% rel. humidity 
Protection Class: IEC 60529 IP 20
Lift: Hand lift or Motor lift 
Height Adjustment Speed: Hand lift models: manual Motor lift models: 25 mm/s 
Height Adjustment: 155 mm 
Drive: brushless DC motor with electronic speed control Heating capacity 1,300 W
Temp. Range Heating Bath: 20 – 210 °C 
Temperature Accuracy Bath: ±1 °C 
Overheat Protection Bath: cut-off at 5 °C over set temperature via separate PT 1000 
Bath Temperature Setting: 5″ LCD color graphic display Heating bath temperature control electronic / digital
Secondary Overtemp. Cut Off: 250 °C 
Material Heating Bath: stainless steel V4A
Diameter Heating Bath: 255 mm 
Volume Heating Bath: 4.5 l 
Display Vapor Temperature: Yes 
Required Accessories For Display Vapor Temperature: Vapor temperature sensor 
Integrated vacuum controller: – 
Timer: Yes 
Timer: Yes 
Programmable Ramps: Yes 
Supply Power: 1,400 W
Max. Size Evaporating Flask: 5 L 
Rotation Speed Range: 10 – 280 rpm 
Solvent Libary: Yes

Thermo Fisher Nanodrop 3300 (OneC Microvolume)

Description: Nanodrop Spectrophotometer
Full spectrum microvolume UV-VIS Measurements with a cuvette option in a single instrument. 
Sampling Options: Pedestal and cuvette
Measures: 1-2ul sample
Dynamic range: (ng/ul dsDNA) 2.0 – 27,500* ng/ul for dsDNA
Full spectra data
Pre-programmed methods for nucleic acids and proteins
Measures nucleic acid: A260
Measures nucleic acid: A260/A230 ratio
Measures purified protein: A280
Measures protein and peptides at: A205
Measures protein: A260/A280 ratio
Preprogrammed methods for colorimetric assays (BCA, Bradford, Lowry. Pierce 660)
Custom methods
Built-in cuvette option
Standalone local control
High-resolution touchscreen interface
Measures fluorescent-labeled nucleic acid and protein samples using absorbance
Autoblank and Automeasure
Acclaro contaminant identification

Licor Odyssey CLx Odyssey

Description: Western Blot Imager
Image Field Size: 25 cm W × 25 cm D (9.8” W × 9.8” D)
Resolution: 21-337 µm
Dynamic Range*: Manual: >4 logs, Auto: >6 logs
Detectors: Silicon avalanche photodiodes
Laser Lifetime: 40,000 hours of operation, Class 1 Laser Product
Light Sources:
Solid-state diode laser at 685 nm
Solid-state diode laser at 785 nm
* This dynamic range is obtained in a single acquisition.
Specifications subject to change without notice.

Evos M5000

Description: Imaging System/ Microscope
Camera: 3.2 Megapixels, Monochrome, CMOS
For Use With (Applications): Imaging
For Use With (Equipment): EVOS™
Light Source: LED
Magnification: 1.25X to 100X
Objectives: 5 Position Turret
Resolution: 1920 x 1080 pixels
Display: 18.5 in Articulated LCD
Dimensions: 18 in (W) x 23 in (H) x 18 in (D)

BioRad CFX Opus 96

Description: RT-PCR (96)
Thermocycler
Maximum Ramp Rate, °C/sec: 5
Average Ramp Rate, °C/sec: 3.3
Heating And Cooling Method: Peltier
Lid, °C: 30–110
Temperature Range, °C: 4–100
Accuracy, °C: ±0.2 of programmed target at 90°C
Uniformity, °C: ±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C: 30–100
Programmable span, °C: 1–24
Optical Detection
Excitation: 6 filtered LEDs
Range of Excitation Wavelengths, nm: 450–684
Range of Emission Wavelengths, nm: 510–730
Sensitivity: Detects 1 copy of target sequence in human genomic DNA
Dynamic Range: 10 orders of magnitude
Scan Time
All Channels, sec: 12
FAM/SYBR® Green Only, sec: 3
Software
Operating Sysetem: Windows 10
Multiplex Analysis: Up to 5 targets per well
System
Sample Capacity, Wells: 96
Sample Size, µl: 1–50 (10–50 recommended)
Communication Interface: USB 2.0

Agilent 8453 UV-Visible Spectrophotometer

Description: UV-VIS
Optical Specifications
Wavelength Range: 190–1100 nm 
Slit Width: 1 nm
EP Resolution Test: >1.6    spectrum of a 0.02% v/v solution of toluene in hexane, ratio absorbance at 269 nm/266 nm 
EP Resolution Test: >1.6    spectrum of a 0.02% v/v solution of toluene in hexane, ratio absorbance at 269 nm/266 nm 
Stray Light: 
<0.03% at 340 nm (NaNO2, ASTM)
<0.05% at 220 nm (NaI, ASTM)
<1% at 200 nm (KCl, EP)
Wavelength Accuracy: <±0.5 nm (NIST 2034)* <±0.2 nm at 486.0 and 656.1 nm 
Wavelength Reproducibility: <±0.02 nm ten consecutive scans (NIST 2034)
Photometric Accuracy: 
<±0.005 A at 1 A (NIST 930e) <±0.01A potassium dichromate, EP method 
Photometric Noise: <0.0002 A sixty 0.5-s scans at 0 A, 500 nm, rms 
Photometric Noise: <0.0002 A sixty 0.5-s scans at 0 A, 500 nm, rms 
Photometric Stability: <0.001 A/h at 0 A, 340 nm, after 1 h warmup, measured over 1 h, every 5 s, constant ambient temperature
Baseline Flatness: <0.001 A 0.5-s blank, 0.5-s scan, rms Typical scan time 1.5 s full range 
Environmental Conditions
Operating Temperature: 0–50 °C (32–122 °F)
Non-Operating Temperature: -40–70 °C (-4-–58 °F) 
Humidity: <95 %, at 25–40 °C (77–104 °F)

BioRad CFX Opus 384

Description: RT-PCR (384)
Thermocycler
Maximum Ramp Rate, °C/sec: 2.5
Average Ramp Rate, °C/sec: 2
Heating And Cooling Method: Peltier
Lid, °C: 30–110
Temperature
Range, °C: 4–100
Accuracy, °C: ±0.2 of programmed target at 90°C
Uniformity, °C: ±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C: 30–100
Programmable span, °C: 1–24
Optical Detection
Excitation: 5 filtered LEDs
Detection: 5 filtered photodiodes
Range of Excitation Wavelengths, nm: 450–650
Range of Emission Wavelengths, nm: 515–690
Sensitivity: Detects 1 copy of target sequence in human genomic DNA
Dynamic Range: 10 orders of magnitude
Scan Time
All Channels, sec: <20
FAM/SYBR® Green Only, sec: 8
System
Sample Capacity, Wells: 384
Sample Size, µl: 1–30 (5–30 recommended)
Communication Interface: USB 2.0

Fisher Scientific HM525 NX

Description: High Performance Routine Cryostat
Ergonomic/Safety features
Mechanical handwheel lock
Optional UV disinfection
Automatic specimen retraction on return stroke
Touch screen operation with intuitive software to accommodate multiple users
Cryochamber features
Chamber cooling to -35°C
27 cryobar storage positions including 4 quick freeze locations
Integrated peltier fast freezing device rapidly cools to -57°C ± 3°C
Programmable and immediate defrost options
Microtome features
Manual light touch rotary flywheel
Section thickness from 1µm to 500µm
Memory function for rapid advance and specimen positioning
Vertical stroke length of 60mm28mm horizontal feed range
40μm specimen retraction on return stroke
XY specimen orientation with 360° z axis rotation
Chamber Temperature: -35°C +/-3°C Max. at +20°C Room Temperature
Defrost: Programmed/ Immediate

Agilent Biotech Synergy Neo2 Hybrid Multi-Mode SMB

Description: Plate Reader
Automation Compatibility: BioStack, BioSpa, BenchCel, 3rd-party
Environment Control: Condensation Control, CO2, O2 Control, 4-Zone Incubation to 70 ºC
Filter Detection Modes: Fluorescence Intensity, Fluorescence Polarization, BRET, Luminescence, TR-FRET, Time Resolved, Fluorescence (Laser), Alpha (Laser)
Imager/Reader Options: Barcode Reader, Reagent Injectors
Instrument Control:  Computer Software
Monochromator Detection Mode: UV-Vis Absorbance, Fluorescence Intentsity (variable bandwidth)
Shaking: Double-orbital, Linear, Orbital
Supported Labware:  6 to 1536-well plates, Take3/Take3 Trio, Petri/Cell culture dishes

Fisher Scientific Heracell 150i Dual SST TC

Description: Tissue Culture Dual Chamber Incubators 
Supported Labware:  6 to 1536-well plates, Take3/Take3 Trio, Petri/Cell culture dishes
Electrical Requirements: 120 V, 50/60 HZ
Electrical Requirements: 120 V, 50/60 HZ
Chamber Material: Copper
CO2 Concentrations Range: 0 to 20%
CO2 Sensor Technology: TC Sensor
Lighting: LED
O2 Concentration Range: Optional 1 to 21% or 5 to 90%
Oxygen Control: Optional 1 to 21% or 5 to 90%
Relative Humidity: <90% at 37°C
Temperature Range (Metric): Ambient +3°C to 55°C
Type: CO2 Incubator
For Use With (Application): General Purpose
Humidity Source: Integrated water reservoir

Nuaire Class II/A2 

Description: Biosafety Cabinets
Class: II Type A2
Air Cleaniliness: ISO-Class-5
Airflow Monitor: Pressure
Cabinet Construction: All welded stainless steel 16/18 GA, Type 304 pressure tight design
Control System:  AeroMax
Downflow FPM (m/s): 60fpm (0.30m/s)
Inflow fpm (m/s): 105 fpm (0.53 m/s)
Protection: Product, Personnel, Environment
Work Access:  8, 10 inches (203, 254 mm) 
Fisher Scientific Isotemp General Purpose Upright
Description: Refrigerator / Freezer Combo
Volts: 115 ~ 
Hz: 60 
AMPS: 6.5 
Phase: 1
Refrigerant Type: R134A

Agilent High-Performance Liquid Chromatography – Analytical 1260 Infinity II_HPLC

Description: HPLC Analysis

m/z Range: n/a

Available detectors: UV-VIS

Sample Loop Size (ul): 100

Column: Poroshell 120 EC-C18, 3.0 x 100mm, 2.7um

Maximum Pressure (bar): 800

Size Of Fraction Collection Vials (ml): n/a

Flow Range (ml/min): 0.001-5

Column thermostat: yes 

Integrated degassing unit: yes

Automatic purge valve: yes

Gradient or isocratic: both

Mass accuracy: n/a

Software: Chemstation

Ion source: n/a

Practices: For targets that have chromophores

Agilent Liquid Chromatography Mass Spectrometry 1290 Infinity II_LC/MSD XT

Description: LC-MS 

m/z Range: 30-2950

Available Detectors: single quad, UV-VIS

Sample Loop Size (ul): 20

Column: Poroshell 120 EC-C18, 2.1x50mm, 1.9um

Maximum Pressure (bar): 1300

Size Of Fraction collection Vials (ml): N/A

Flow Range (ml/min): 0.001-5

Column Thermostat: yes 

Integrated Degassing Unit: yes

Automatic Purge Valve: yes

Gradient or Isocratic: both

Mass Accuracy: nominal

Software: Chemstation

Ion Source: ESI

Practices: For polar, acidic, & basic molecules

Agilent High-Performance Liquid Chromatography – Preparative 1260 Infinity II_FC-AS

Description: HPLC (Prep)

m/z Range: n/a

Available Detectors: UV-VIS

Sample Loop Size (ul): 100

Column: Poroshell 120 EC-C18, 4.6 x 100mm, 2.7um

Maximum Pressure (bar): 600

Size Of Fraction Collection Vials (ml): 2

Flow Range (ml/min): 0.001-5

Column Thermostat: yes 

Integrated Degassing Unit: yes

Automatic Purge Valve: no, manual

Gradient or Isocratic: both

Mass Accuracy: n/a

Software: Chemstation

Ion Source: n/a

Practices: For targets that have chromophores

Agilent Gel Permeation Chromatography 1260 Infinity II_GPC/SEC MDS

Description: GPC
m/z Range: 200-10M
Available Detectors: LS, RID, viscometer
Sample Loop Size (ul): 100
Column: none
Maximum Pressure (bar): 600
Size Of Fraction Collection Vials (ml): N/A
Flow Range (ml/min): 0.001-5
Column Thermostat: yes 
Integrated Degassing Unit: yes
Automatic Purge Valve: no, manual
Gradient or Isocratic: isocratic only
Mass Accuracy: average
Software: GPC/SEC
Ion Source: n/a
Practices: For macromolecules
Comment: for both organic & aqueous
Concern: extended time for solvent transfer and equilibration

Agilent Gas Chromatography-Mass Spectrometry 8890 GC/5977B MSD

Description: GC-MS
m/z Range: 1.6 – 1050
Available Detectors: single quad
Sample Loop Size (ul): n/a
Column: HP-5MS UI, 30m x 0.25mm ID x 0.25uM film
Maximum Pressure (bar): n/a
Size Of Fraction Collection Vials (ml): n/a
Flow Range (ml/min): n/a
Column Thermostat: yes
Integrated Degassing Unit: n/a
Automatic Purge Valve: n/a
Gradient or Isocratic: both
Mass Accuracy: nominal
Software: MassHunter
Ion Source: EI
Practices: For volatile targets
Accessories: headspace sampler (HS)
Comments: Use is only for users with previous experience
Concerns: Worldwide helium shortage, no NIST database, HS sampler consumes much helium. 
EMD Millipore Milli-Q Reference
Description: Water Purification System
Conductivity: <100ppb
Contaminant Rejection (Microbiological): Less than 1cfu/ml
Feedwater Source: Elix Reverse Osmosis, Deionized, Distilled Water
Flow Rate: 50 and 2000 ml/min
Product Water Type: Type 1

Perkin Elmer In Vivo Imaging System (IVIS) Inst. Spectrum 120V Andor Camera

Description: IVIS Spectrum
High-sensitivity in vivo imaging of fluorescence and bioluminescence
High-sensitivity in vivo imaging of fluorescence and bioluminescence
High throughput (5 mice) with 23 cm field of view
High resolution (to 20 microns) with 3.9 cm field of view
Twenty eight high efficiency filters spanning 430 – 850 nm
Supports spectral unmixing applications
Ideal for distinguishing multiple bioluminescent and fluorescent reporters
Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
NIST traceable absolute calibrations
Gas anesthesia inlet and outlet ports
Class I Laser Product